Journal: The Journal of Clinical Investigation

Article Title: CDK12/13 inactivation triggers STING-mediated antitumor immunity in preclinical models

doi: 10.1172/JCI193745

Figure Lengend Snippet: ( A ) Enrichment of the indicated pathways in Myc-CaP cells treated with YJ1206 at 1 μM for 24 hours. Adj., adjusted. ( B ) Immunoblot of the indicated proteins in Myc-CaP cells treated with YJ1206 at increasing concentrations for 4 hours, or siRNAs targeting Cdk12 and/or Cdk13 . Nontargeting siRNA was used as control. GAPDH was used as a loading control. ( C ) Top: Analysis of the indicated gene expression by RT-qPCR in Myc-CaP cells treated with YJ1206 at 1 μM for 15 hours, or siRNAs targeting Cdk12 and/or Cdk13 . Nontargeting siRNA was used as control. Bottom left: IFN-β ELISA results in Myc-CaP cells treated as described above. Bottom right: Flow cytometry assessing surface MHC-I expression in Myc-CaP cells treated as described above. ( D ) Immunoblot of the noted proteins in B16-F10 cells treated with YJ1206 at increasing concentrations for 4 hours. ( E and F ) Flow cytometry median fluorescence intensity (MFI) quantifications of surface MHC-I ( E ) or PD-L1 ( F ) in the indicated cells treated with YJ1206 at 1 μM or 3 μM for 15 hours. ( G ) Quantification of immunofluorescence DNA/RNA hybrid (red) staining in Myc-CaP cells treated with 1 μM YJ1206 for 4 hours or siRNA targeting Cdk12 and/or Cdk13 , with/without RNase H. Representative images are in . Nontargeting siRNA was used as control. Forty (siRNA treatment) or 20 (YJ1206 or DMSO treatment) cells were used per data point. Data in C , E , and F are displayed as mean ± SD of triplicate experiments. Data in G are presented as box-and-whisker plots, with the median (center line), 25th–75th percentiles (box), and minimum to maximum values (whiskers). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 2-tailed t test. NS, not significant. Bonferroni’s correction was applied for multiple comparisons in C and E – G .

Article Snippet: IFN-β ELISA (R&D Systems, DY8234-05 and DY008B) and cGAMP ELISA (Cayman Chemical, 501700) kits were used per the manufacturer’s protocols.

Techniques: Western Blot, Control, Gene Expression, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Expressing, Fluorescence, Immunofluorescence, Staining, Whisker Assay

Journal: The Journal of Clinical Investigation

Article Title: CDK12/13 inactivation triggers STING-mediated antitumor immunity in preclinical models

doi: 10.1172/JCI193745

Figure Lengend Snippet: ( A and B ) Representative images ( A ) or quantification ( B ) of immunofluorescence assessing PCNA-RNAPII PLA foci in Myc-CaP cells treated with siRNA targeting Cdk12 and/or Cdk13 , or YJ1206 at 3 μM for 4 hours, with or without DRB or triptolide treatment. ( C – E ) Representative images ( C ) or quantification ( D and E ) of dsDNA ( C , left, and D ) and ssDNA ( C , right, and E ) in Myc-CaP cells treated with 3 μM YJ1206 for 4 hours, with or without DRB treatment. Scale bars: 5 μm. ( F ) ELISA measuring cGAMP levels in Myc-CaP cells treated with YJ1206 at the indicated concentrations. ( G ) Immunoblot of the noted proteins in Myc-CaP cells treated with YJ1206 at 1 μM, with or without DRB for 4 hours. Data in D and E are presented as box-and-whisker plots, with the median (center line), 25th–75th percentiles (box), and minimum to maximum values (whiskers). Data are displayed as mean ± SEM in F of triplicate experiments. One hundred cells were used per data point in B , D , and E . **** P < 0.0001 by 2-tailed t test. NS, not significant. Bonferroni’s correction was applied for multiple comparisons.

Article Snippet: IFN-β ELISA (R&D Systems, DY8234-05 and DY008B) and cGAMP ELISA (Cayman Chemical, 501700) kits were used per the manufacturer’s protocols.

Techniques: Immunofluorescence, Enzyme-linked Immunosorbent Assay, Western Blot, Whisker Assay

Journal: bioRxiv

Article Title: Adipose Tissue Inflammation, Oxidative Stress, and Altered Adipogenesis Are Drivers of Dyslipidemia – A Multi-Omics Overview of Dyslipidemia in Obesity

doi: 10.1101/2025.10.15.682716

Figure Lengend Snippet: Correlation plots of leptin with HDL-C and ApoAI with lines of best fit and confidence interval. Spearman’s rank correlation (r), and its Benjamini-Hochberg adjusted p-value ( p ), only significant correlations depicted.

Article Snippet: Plasma adiponectin and leptin concentrations were measured using human Adiponectin/Acrp30 DuoSet ® ELISA (Y1065-05; R&D Systems, Minneapolis, USA) and human Leptin DuoSet ® ELISA (DY398-05; R&D Systems, Minneapolis, USA).

Techniques:

Journal: Life sciences

Article Title: Effects of chitinase-1 inhibitor in obesity-induced and -aggravated asthma in a murine model

doi: 10.1016/j.lfs.2023.122163

Figure Lengend Snippet: TGF- β (A), IL-1 β (B), IL-6 (C), TNF- α (D), IL-13 (E), and IL-4 (F) levels in lung homogenates Bar and line graphs present mean ± standard error. Cht, chitin; CPX, chitinase-1 inhibitor; OVA, ovalbumin * P < 0.05 between them analyzed by one-way ANOVA followed by post-hoc Bonferroni test.

Article Snippet: The concentrations of chitinase-1 (Fine biotech, EM0939, Wuhan, China), hydroxyproline (Biovision, K555–100, CA, USA), collagen-1 (Fine biotech, EM6446), interleukin (IL)-1 β (DY401), TNF- α (DY410), IL-4 (DY404), IL-6 (DY406), IL-13 (DY413), and TGF- β (DY1679) in lung homogenates were assessed using an enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, San Diego, USA) according to the manufacturer’s instructions.

Techniques:

Journal: Life sciences

Article Title: Effects of chitinase-1 inhibitor in obesity-induced and -aggravated asthma in a murine model

doi: 10.1016/j.lfs.2023.122163

Figure Lengend Snippet: Pathologic findings (H&E, PAS, and MT; all × 200 magnification) (A), quantitative fibrosis area (B), level of hydroxyproline in lung homogenates (C), and level of collagen-1 in lung homogenates (D). TGF- β (E), TNF- α (F), IL-1 β (G), IL-6 (H), IL-4 (I), and IL-13 (J) levels in lung homogenates Bar and line graphs present mean ± standard error. Cht, chitin; CPX, chitinase-1 inhibitor; HFD, high-fat diet; OVA, ovalbumin; H&E, hematoxylin and eosin; PAS, periodic acid-Schiff; MT, Masson’s trichrome * P < 0.05 between them analyzed by one-way ANOVA followed by post-hoc Bonferroni test.

Article Snippet: The concentrations of chitinase-1 (Fine biotech, EM0939, Wuhan, China), hydroxyproline (Biovision, K555–100, CA, USA), collagen-1 (Fine biotech, EM6446), interleukin (IL)-1 β (DY401), TNF- α (DY410), IL-4 (DY404), IL-6 (DY406), IL-13 (DY413), and TGF- β (DY1679) in lung homogenates were assessed using an enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, San Diego, USA) according to the manufacturer’s instructions.

Techniques: